Grants/Awards
Funded, 2021
- U01, NIAID, Leveraging an ongoing longitudinal study of influenza vaccination to define immune signatures of response and risk of infection in older adults >75. PI: Sean Leng, Jay Bream
Funded, 2020
- U54, NIA, Sex and Age Differences in Immunity to Influenza (SADII). PI: Sabra Klein
- R21, NIA, T-cell responses to CMV as a determinant of systemic immune activation. PI: Joseph Margolick, Sean Leng
- R42, NIA, A Randomized, Blinded, Placebo-Controlled Clinical Trial to Evaluate Longeveron Mesenchymal Stem Cell (LMSC) Therapy for Treating The Metabolic Syndrome. PI: Joshua Hare, Sean Leng, Andrew Olivia
Funded, 2017 or Earlier
- R01, NIAID, Influence Vaccine Failure in Adults Over Age 75: Role of Chronic CMV Infection. PI: Sean X Leng
- R21, NIA, Chronic CMV infection in the elderly: diagnosis and link to chronic inflammation. PI: Sean Leng
- R21, NIA, Inflamation-Gene Expression by Monocytes in Frailty. PI: Sean Leng
- K23, NIA, Vaccine-Induced Immunity against Influenza in Frailty. PI: Sean Leng
- Paul Beeson Career Development Award in Aging Research, 2006
Publications
2023
Wunderlich B, Laskow T, Li H, Zhang L, Abrams E, Tian J, Yu J, Chen Y, Tavenier J, Huang Y, Talaat K, Bream JH, Xue QL, Pawelec G, Leng SX. Interseason waning of vaccine-induced hemagglutination inhibition antibody titers and contributing factors to pre-existing humoral immunity against influenza in community-dwelling older adults 75 years and older. Immun Ageing. 2023 Jul 31;20(1):38. doi: 10.1186/s12979-023-00362-8. PMID: 37525151; PMCID: PMC10388475.
Shapiro JR, Seddu K, Park HS, Lee JS, Creisher PS, Yin A, Shea P, Kuo H, Li H, Abrams E, Leng SX, Morgan R, Klein SL. The intersection of biological sex and gender in adverse events following seasonal influenza vaccination in older adults. Res Sq [Preprint]. 2023 Feb 8:rs.3.rs-2557775. doi: 10.21203/rs.3.rs-2557775/v1. Update in: Immun Ageing. 2023 Aug 29;20(1):43. PMID: 36798418; PMCID: PMC9934749.
Shapiro JR, Privor-Dumm L, Rosser EN, Leng SX, Klein SL, Morgan R. The intersection of gender and race in older adults’ decision to receive COVID-19 vaccines. Vaccine. 2023 Jan 4;41(1):211-218. doi: 10.1016/j.vaccine.2022.09.043. Epub 2022 Sep 20. PubMed PMID: 36435705; PubMed Central PMCID: PMC9485425.
2022
Huang BW, Guo PH, Liu JZ, Leng SX, Wang L. Investigating adolescent mental health of Chinese students during the COVID-19 pandemic: Multicenter cross-sectional comparative investigation. World J Psychiatry. 2022 Nov 19;12(11):1323-1334. doi: 10.5498/wjp.v12.i11.1323. eCollection 2022 Nov 19. PubMed PMID: 36438680; PubMed Central PMCID: PMC9693833.
Leng SX, Pawelec G. Single-cell immune atlas for human aging and frailty. Life Med. 2022 Oct;1(2):67-70. doi: 10.1093/lifemedi/lnac013. eCollection 2022 Oct. PubMed PMID: 36699943; PubMed Central PMCID: PMC9869752.
Zhang W, Li H, Bream JH, Nilles TL, Leng SX, Margolick JB. Longitudinal association of cytokine-producing CMV-specific T cells with frailty in HIV-infected and -uninfected men who have sex with men. Immun Ageing. 2022 Mar 7;19(1):13. doi: 10.1186/s12979-022-00270-3. PubMed PMID: 35255947; PubMed Central PMCID: PMC8900335.
Shapiro JR, Sitaras I, Park HS, Aytenfisu TY, Caputo C, Li M, Lee J, Johnston TS, Li H, Wouters C, Hauk P, Jacobsen H, Li Y, Abrams E, Yoon S, Kocot AJ, Yang T, Huang Y, Cramer SM, Betenbaugh MJ, Debes AK, Morgan R, Milstone AM, Karaba AH, Pekosz A, Leng SX, Klein SL. Association of Frailty, Age, and Biological Sex With Severe Acute Respiratory Syndrome Coronavirus 2 Messenger RNA Vaccine-Induced Immunity in Older Adults. Clin Infect Dis. 2022 Aug 15;75(Suppl 1):S61-S71. doi: 10.1093/cid/ciac397. PubMed PMID: 35607747; PubMed Central PMCID: PMC9376280.
Kini A, Morgan R, Kuo H, Shea P, Shapiro J, Leng SX, Pekosz A, Klein SL. Differences and disparities in seasonal influenza vaccine, acceptance, adverse reactions, and coverage by age, sex, gender, and race. Vaccine. 2022 Mar 8;40(11):1643-1654. doi: 10.1016/j.vaccine.2021.04.013. Epub 2021 Apr 28. Review. PubMed PMID: 33933316; PubMed Central PMCID: PMC8551304.
Althoff KN, Stewart CN, Humes E, Zhang J, Gerace L, Boyd CM, Wong C, Justice AC, Gebo KA, Thorne JE, Rubtsova AA, Horberg MA, Silverberg MJ, Leng SX, Rebeiro PF, Moore RD, Buchacz K, Kasaie P. The shifting age distribution of people with HIV using antiretroviral therapy in the United States. AIDS. 2022 Mar 1;36(3):459-471. doi: 10.1097/QAD.0000000000003128. PubMed PMID: 34750289; PubMed Central PMCID: PMC8795488.
Shapiro JR, Morgan R, Leng SX, Klein SL. Roadmap for Sex-Responsive Influenza and COVID-19 Vaccine Research in Older Adults. Front Aging. 2022;3:836642. doi: 10.3389/fragi.2022.836642. eCollection 2022. Review. PubMed PMID: 35821800; PubMed Central PMCID: PMC9261334.
2021
Shapiro JR, Li H, Morgan R, Chen Y, Kuo H, Ning X, Shea P, Wu C, Merport K, Saldanha R, Liu S, Abrams E, Chen Y, Kelly DC, Sheridan-Malone E, Wang L, Zeger SL, Klein SL, Leng SX. Sex-specific effects of aging on humoral immune responses to repeated influenza vaccination in older adults. NPJ Vaccines. 2021 Dec 9;6(1):147. doi: 10.1038/s41541-021-00412-6. PubMed PMID: 34887436; PubMed Central PMCID: PMC8660902.
Li P, Xin Q, Hui J, Yuan R, Wang Y, Miao Y, Lee SM, Leng SX, Cong W. Efficacy and Safety of Tongxinluo Capsule as Adjunctive Treatment for Unstable Angina Pectoris: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Front Pharmacol. 2021;12:742978. doi: 10.3389/fphar.2021.742978. eCollection 2021. Review. PubMed PMID: 34707500; PubMed Central PMCID: PMC8544810.
Zhang W, Bream JH, Leng SX, Margolick JB. Validation of Preamplification to Improve Quantification of Cytomegalovirus DNA Using Droplet Digital Polymerase Chain Reaction. Anal Chem. 2021 Mar 2;93(8):3710-3716. doi: 10.1021/acs.analchem.0c02890. Epub 2021 Feb 17. PubMed PMID: 33596050; PubMed Central PMCID: PMC10074994.
Leng SX, Kittleson MM. Beyond the eyeball test: Impact and potential mechanisms of frailty in heart transplant candidates. J Heart Lung Transplant. 2021 Feb;40(2):95-98. doi: 10.1016/j.healun.2020.12.004. Epub 2021 Jan 5. PubMed PMID: 33419640.
Chen Y, Klein SL, Garibaldi BT, Li H, Wu C, Osevala NM, Li T, Margolick JB, Pawelec G, Leng SX. Aging in COVID-19: Vulnerability, immunity and intervention. Ageing Res Rev. 2021 Jan;65:101205. doi: 10.1016/j.arr.2020.101205. Epub 2020 Oct 31. Review. PubMed PMID: 33137510; PubMed Central PMCID: PMC7604159.
2020
Cheng Y, Cao X, Cao Z, Xu C, Sun L, Gao Y, Wang Y, Li S, Wu C, Li X, Wang Y, Leng SX. Effects of influenza vaccination on the risk of cardiovascular and respiratory diseases and all-cause mortality. Ageing Res Rev. 2020 Sep;62:101124. doi: 10.1016/j.arr.2020.101124. Epub 2020 Jul 16. Review. PubMed PMID: 32683040; PubMed Central PMCID: PMC7365105.
Li X, Leng SX. Influenza immunization among Chinese seniors: Urgent calling for improving vaccination coverage, education, and research. Aging Med (Milton). 2020 Mar;3(1):12-15. doi: 10.1002/agm2.12103. eCollection 2020 Mar. PubMed PMID: 32232187; PubMed Central PMCID: PMC7099750.
Leng SX, Margolick JB. Aging, sex, inflammation, frailty, and CMV and HIV infections. Cell Immunol. 2020 Feb;348:104024. doi: 10.1016/j.cellimm.2019.104024. Epub 2019 Nov 27. Review. PubMed PMID: 31843200; PubMed Central PMCID: PMC7002257.
2019
Zheng L, Li H, Fu L, Liu S, Yan Q, Leng SX. Blocking cellular N-glycosylation suppresses human cytomegalovirus entry in human fibroblasts. Microb Pathog. 2020 Jan;138:103776. doi: 10.1016/j.micpath.2019.103776. Epub 2019 Oct 7. PubMed PMID: 31600539.
Fang F, Wang N, Yan S, Wang L, Lu Y, Li J, Li H, Pham G, Li C, Leng SX, Tian H. Impaired glucose tolerance predicts all-cause mortality among older men at high risk for cardiovascular disease in China. Prim Care Diabetes. 2019 Dec;13(6):495-504. doi: 10.1016/j.pcd.2019.01.004. Epub 2019 Mar 9. PubMed PMID: 30862424.
Leng SX, Liu B, Kennedy BK. Aging mechanisms and interventions that impact senior health: Introduction to a special issue on Geroscience. Aging Med (Milton). 2019 Sep;2(3):126-129. doi: 10.1002/agm2.12086. eCollection 2019 Sep. PubMed PMID: 31942523; PubMed Central PMCID: PMC6880738.
Leng SX, Li XY. Introduction of Abstracts to be presented at the Eighth Chinese Congress on Gerontology and Health Industry. J Am Geriatr Soc. 2019 Sep;67(S4):S593-S594. doi: 10.1111/jgs.16119. PubMed PMID: 31454412.
Tavenier J, Margolick JB, Leng SX. T-cell immunity against cytomegalovirus in HIV infection and aging: relationships with inflammation, immune activation, and frailty. Med Microbiol Immunol. 2019 Aug;208(3-4):289-294. doi: 10.1007/s00430-019-00591-z. Epub 2019 Mar 21. Review. PubMed PMID: 30900090; PubMed Central PMCID: PMC6635075.
Tavenier J, Leng SX. Inflammatory Pathways to Anemia in the Frail Elderly. Clin Geriatr Med. 2019 Aug;35(3):339-348. doi: 10.1016/j.cger.2019.03.005. Epub 2019 May 9. Review. PubMed PMID: 31230735.
2018
Li X, Fan L, Leng SX. Reply to: Comments on the Aging Tsunami and Senior Healthcare Development in China. J Am Geriatr Soc. 2019 Feb;67(2):405. doi: 10.1111/jgs.15652. Epub 2018 Nov 19. PubMed PMID: 30452081.
Li X, Fan L, Leng SX. Reply to: Update on Aging Statistics and Geriatrics Development in China. J Am Geriatr Soc. 2019 Jan;67(1):188-189. doi: 10.1111/jgs.15605. Epub 2018 Oct 18. PubMed PMID: 30335189.
Margolick JB, Bream JH, Nilles TL, Li H, Langan SJ, Deng S, Wang R, Wada N, Leng SX. Relationship Between T-Cell Responses to CMV, Markers of Inflammation, and Frailty in HIV-uninfected and HIV-infected Men in the Multicenter AIDS Cohort Study. J Infect Dis. 2018 Jun 20;218(2):249-258. doi: 10.1093/infdis/jiy005. PubMed PMID: 29529309; PubMed Central PMCID: PMC6009694.
Keshtkar-Jahromi M, Ouyang M, Keshtkarjahromi M, Almed S, Li H, Walston JD, Rios R, Leng SX. Effect of influenza vaccine on tumor necrosis factor-like weak inducer of apoptosis (TWEAK) in older adults. Vaccine. 2018 Apr 12;36(16):2220-2225. doi: 10.1016/j.vaccine.2017.11.045. Epub 2018 Mar 13. PubMed PMID: 29548604; PubMed Central PMCID: PMC5889121.
2017
Leng SX, Kamil J, Purdy JG, Lemmermann NA, Reddehase MJ, Goodrum FD. Recent advances in CMV tropism, latency, and diagnosis during aging. Geroscience. 2017 Jun;39(3):251-259. doi: 10.1007/s11357-017-9985-7. Epub 2017 Jul 5. PubMed PMID: 28681110; PubMed Central PMCID: PMC5505884.
Zhang X, Meng X, Chen Y, Leng SX, Zhang H. The Biology of Aging and Cancer: Frailty, Inflammation, and Immunity. Cancer J. 2017 Jul/Aug;23(4):201-205. doi: 10.1097/PPO.0000000000000270. Review. PubMed PMID: 28731941.
Piggott DA, Muzaale AD, Varadhan R, Mehta SH, Westergaard RP, Brown TT, Patel KV, Walston JD, Leng SX, Kirk GD. Frailty and Cause-Specific Hospitalization Among Persons Aging With HIV Infection and Injection Drug Use. J Gerontol A Biol Sci Med Sci. 2017 Mar 1;72(3):389-394. doi: 10.1093/gerona/glw142. PubMed PMID: 27516622; PubMed Central PMCID: PMC6075460.
2016
Mao G, Li H, Ding X, Meng X, Wang G, Leng SX. Suppressive effects of sirtinol on human cytomegalovirus (hCMV) infection and hCMV-induced activation of molecular mechanisms of senescence and production of reactive oxygen species. Mech Ageing Dev. 2016 Sep;158:62-9. doi: 10.1016/j.mad.2015.12.005. Epub 2016 Jan 4. PubMed PMID: 26763147; PubMed Central PMCID: PMC5935109.
Xue QL, Yang H, Li HF, Abadir PM, Burks TN, Koch LG, Britton SL, Carlson J, Chen L, Walston JD, Leng SX. Rapamycin increases grip strength and attenuates age-related decline in maximal running distance in old low capacity runner rats. Aging (Albany NY). 2016 Apr;8(4):769-76. doi: 10.18632/aging.100929. PubMed PMID: 26997106; PubMed Central PMCID: PMC4925827.
2015
Piggott DA, Varadhan R, Mehta SH, Brown TT, Li H, Walston JD, Leng SX, Kirk GD. Frailty, Inflammation, and Mortality Among Persons Aging With HIV Infection and Injection Drug Use. J Gerontol A Biol Sci Med Sci. 2015 Dec;70(12):1542-7. doi: 10.1093/gerona/glv107. Epub 2015 Sep 18. PubMed PMID: 26386010; PubMed Central PMCID: PMC4643614.
Leng SX, Dandorf S, Li H, Carlson J, Hui J, Mehta SH, Piggott D, Islam S, Manwani B, Kirk GD. Associations of Circulating Soluble Tumor Necrosis Factor-α Receptors 1 and 2 with Interleukin-6 Levels in an Aging Cohort of Injection Drug Users with or at High Risk for HIV Infection. AIDS Res Hum Retroviruses. 2015 Dec;31(12):1257-64. doi: 10.1089/AID.2015.0134. Epub 2015 Oct 13. PubMed PMID: 26414536; PubMed Central PMCID: PMC4663639.
Najarro K, Nguyen H, Chen G, Xu M, Alcorta S, Yao X, Zukley L, Metter EJ, Truong T, Lin Y, Li H, Oelke M, Xu X, Ling SM, Longo DL, Schneck J, Leng S, Ferrucci L, Weng NP. Telomere Length as an Indicator of the Robustness of B- and T-Cell Response to Influenza in Older Adults. J Infect Dis. 2015 Oct 15;212(8):1261-9. doi: 10.1093/infdis/jiv202. Epub 2015 Mar 31. PubMed PMID: 25828247; PubMed Central PMCID: PMC4577042.
Li H, Mao G, Carlson J, Leng SX. A novel flow cytometry-based tool for determining the efficiency of human cytomegalovirus infection in THP-1 derived macrophages. J Virol Methods. 2015 Sep 1;221:127-30. doi: 10.1016/j.jviromet.2015.04.028. Epub 2015 May 7. PubMed PMID: 25958130; PubMed Central PMCID: PMC5960983.
Leng SX, Margolick JB. Understanding frailty, aging, and inflammation in HIV infection. Curr HIV/AIDS Rep. 2015 Mar;12(1):25-32. doi: 10.1007/s11904-014-0247-3. Review. PubMed PMID: 25656346; PubMed Central PMCID: PMC4751047.
2014
Li H, Margolick JB, Bream JH, Nilles TL, Langan S, Bui HT, Sylwester AW, Picker LJ, Leng SX. Heterogeneity of CD4+ and CD8+ T-cell responses to cytomegalovirus in HIV-infected and HIV-uninfected men who have sex with men. J Infect Dis. 2014 Aug 1;210(3):400-4. doi: 10.1093/infdis/jiu093. Epub 2014 Feb 14. PubMed PMID: 24532602; PubMed Central PMCID: PMC4110456.
Li H, Weng P, Najarro K, Xue QL, Semba RD, Margolick JB, Leng SX. Chronic CMV infection in older women: longitudinal comparisons of CMV DNA in peripheral monocytes, anti-CMV IgG titers, serum IL-6 levels, and CMV pp65 (NLV)-specific CD8(+) T-cell frequencies with twelve year follow-up. Exp Gerontol. 2014 Jun;54:84-9. doi: 10.1016/j.exger.2014.01.010. Epub 2014 Jan 15. PubMed PMID: 24440388; PubMed Central PMCID: PMC3989432.
2013
Salter ML, Lau B, Mehta SH, Go VF, Leng S, Kirk GD. Correlates of elevated interleukin-6 and C-reactive protein in persons with or at high risk for HCV and HIV infections. J Acquir Immune Defic Syndr. 2013 Dec 15;64(5):488-95. doi: 10.1097/QAI.0b013e3182a7ee2e. PubMed PMID: 23978997; PubMed Central PMCID: PMC3848037.
Piggott DA, Muzaale AD, Mehta SH, Brown TT, Patel KV, Leng SX, Kirk GD. Frailty, HIV infection, and mortality in an aging cohort of injection drug users. PLoS One. 2013;8(1):e54910. doi: 10.1371/journal.pone.0054910. Epub 2013 Jan 31. PubMed PMID: 23382997; PubMed Central PMCID: PMC3561408.
2011
Leng SX, Qu T, Semba RD, Li H, Yao X, Nilles T, Yang X, Manwani B, Walston JD, Ferrucci L, Fried LP, Margolick JB, Bream JH. Relationship between cytomegalovirus (CMV) IgG serology, detectable CMV DNA in peripheral monocytes, and CMV pp65(495-503)-specific CD8+ T cells in older adults. Age (Dordr). 2011 Dec;33(4):607-14. doi: 10.1007/s11357-011-9205-9. Epub 2011 Jan 28. PubMed PMID: 21274637; PubMed Central PMCID: PMC3220402.
Leng SX. Role of chronic cytomegalovirus infection in T-cell immunosenescence and frailty: more questions than answers. J Am Geriatr Soc. 2011 Dec;59(12):2363-5. doi: 10.1111/j.1532-5415.2011.03815.x. PubMed PMID: 22188078.
Leng SX, Li H, Xue QL, Tian J, Yang X, Ferrucci L, Fedarko N, Fried LP, Semba RD. Association of detectable cytomegalovirus (CMV) DNA in monocytes rather than positive CMV IgG serology with elevated neopterin levels in community-dwelling older adults. Exp Gerontol. 2011 Aug;46(8):679-84. doi: 10.1016/j.exger.2011.04.002. Epub 2011 Apr 13. PubMed PMID: 21513787; PubMed Central PMCID: PMC3134158.
Leng SX, Tian X, Matteini A, Li H, Hughes J, Jain A, Walston JD, Fedarko NS. IL-6-independent association of elevated serum neopterin levels with prevalent frailty in community-dwelling older adults. Age Ageing. 2011 Jul;40(4):475-81. doi: 10.1093/ageing/afr047. Epub 2011 May 17. PubMed PMID: 21586579; PubMed Central PMCID: PMC3114624.